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產品名稱：重組大鼠成纖維細胞生長因子21蛋白
產品型號：rRtFGF-21
產品廠商：KALANG
產品文檔：

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簡單介紹：

重組大鼠成纖維細胞生長因子21蛋白與其它公司提供的重組蛋白不同，rRtFGF-21蛋白產品為采用CFS的無細胞麥胚蛋白合成系統表達出來的重組蛋白，可表達出對細胞有毒性、易被蛋白酶降解的蛋白；并獲得具有良好的可溶性，并有翻譯后修飾、從而部分具有功能的蛋白.同時獨有的全自動蛋白純化技術則簡便高效，將蛋白純化過程中對蛋白的損傷降低到*小程度.重組大鼠成纖維細胞生長因子21蛋白（全長序列）產品可用于Western Blot驗證、抗體制備、蛋白檢測、ELISA等試驗中.

詳情介紹：

重組大鼠成纖維細胞生長因子21蛋白

Species Rat

Accession Q8VI80

GeneID 170580

Source Escherichia coli.

Molecular Weight 重組大鼠成纖維細胞生長因子21蛋白Approximately 19.7 kDa, a single non-glycosylated polypeptide chain containing 180 amino acids.

Quantity 5μg/25μg/1000μg

AA Sequence AYPISDSSPL LQFGGQVRQR YLYTDDDQDT EAHLEIREDG TVVGTAHRSP ESLLELKALK PGVIQILGVK ASRFLCQQPD GTLYGSPHFD PEACSFRELL LKDGYNVYQS EAHGLPLRLP QKDSQDPATR GPVRFLPMPG LPHEPQEQPG VLPPEPPDVG SSDPLSMVEP LQGRSPSYAS

Purity > 95 % by SDS-PAGE and HPLC analyses.

Biological Activity 重組大鼠成纖維細胞生長因子21蛋白Fully biologically active when compared to standard. The ED₅₀ as determined by thymidine uptake assay using FGF-receptors transfected BaF3 cells is less than 0.5 μg/ml, corresponding to a specific activity of > 2.0 × 10³ IU/mg in the presence of 5 μg/ml of rMuKlotho-β and 10 μg/ml of heparin.

Physical Appearance White lyophilized powder.

Formulation Lyophilized from a 0.2 μm filtered concentrated solution in PBS, 500 mM NaCl, pH 7.4.

Endotoxin 重組大鼠成纖維細胞生長因子21蛋白Less than 1 EU/μg of rRtFGF-21 as determined by LAL method.

Reconstitution We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Reconstitute in sterile distilled water or aqueous buffer containing 0.1 % BSA to a concentration of 0.1-1.0 mg/mL. Stock solutions should be apportioned into working aliquots and stored at ≤ -20 °C. Further dilutions should be made in appropriate buffered solutions.

Storage This lyophilized preparation is stable at 2-8 °C, but should be kept at -20 °C for long term storage, preferably desiccated. Upon reconstitution, the preparation is stable for up to one week at 2-8 °C. For maximal stability, apportion the reconstituted preparation into working aliquots and store at -20 °C to -70 °C. Avoid repeated freeze/thaw cycles.

重組大鼠成纖維細胞生長因子21蛋白

SDS-PAGE

Reference 1. Nishimura T, Nakatake Y, Konishi M, et al. 2000. Biochim Biophys Acta. 1492:203-6.
2. Kharitonenkov A, Shiyanova TL, Koester A, et al. 2005. J Clin Invest. 115:1627-35.
3. Kurosu H, Choi M, Ogawa Y, et al. 2007. J Biol Chem. 282:26687-95

Background Fibroblast growth factor 21 (FGF-21) encoded by the FGF-21 gene belongs to the large FGF family and it is specifically induced by HMGCS2 activity. In mice, brown adipose tissue becomes a source of systemic FGF-21 after cold exposure. FGF-21 stimulates glucose uptake in differentiated adipocytes via the induction of glucose transporter SLC2A1/GLUT1 expression (but not SLC2A4/GLUT4 expression) and the activity depends on the presence of KLB. Recombinant rat FGF-21 contains 180 amino acids residues and show limited binding to heparin. In addition, rat FGF-21 respectively shows 81 % and 92 % a.a. identity to human and murine FGF-21, and it show activity on murine and human cells.

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Species	Rat
Accession	Q8VI80
GeneID	170580
Source	Escherichia coli.
Molecular Weight	重組大鼠成纖維細胞生長因子21蛋白Approximately 19.7 kDa, a single non-glycosylated polypeptide chain containing 180 amino acids.
Quantity	5μg/25μg/1000μg
AA Sequence	AYPISDSSPL LQFGGQVRQR YLYTDDDQDT EAHLEIREDG TVVGTAHRSP ESLLELKALK PGVIQILGVK ASRFLCQQPD GTLYGSPHFD PEACSFRELL LKDGYNVYQS EAHGLPLRLP QKDSQDPATR GPVRFLPMPG LPHEPQEQPG VLPPEPPDVG SSDPLSMVEP LQGRSPSYAS
Purity	> 95 % by SDS-PAGE and HPLC analyses.
Biological Activity	重組大鼠成纖維細胞生長因子21蛋白Fully biologically active when compared to standard. The ED₅₀ as determined by thymidine uptake assay using FGF-receptors transfected BaF3 cells is less than 0.5 μg/ml, corresponding to a specific activity of > 2.0 × 10³ IU/mg in the presence of 5 μg/ml of rMuKlotho-β and 10 μg/ml of heparin.
Physical Appearance	White lyophilized powder.
Formulation	Lyophilized from a 0.2 μm filtered concentrated solution in PBS, 500 mM NaCl, pH 7.4.
Endotoxin	重組大鼠成纖維細胞生長因子21蛋白Less than 1 EU/μg of rRtFGF-21 as determined by LAL method.
Reconstitution	We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Reconstitute in sterile distilled water or aqueous buffer containing 0.1 % BSA to a concentration of 0.1-1.0 mg/mL. Stock solutions should be apportioned into working aliquots and stored at ≤ -20 °C. Further dilutions should be made in appropriate buffered solutions.
Storage	This lyophilized preparation is stable at 2-8 °C, but should be kept at -20 °C for long term storage, preferably desiccated. Upon reconstitution, the preparation is stable for up to one week at 2-8 °C. For maximal stability, apportion the reconstituted preparation into working aliquots and store at -20 °C to -70 °C. Avoid repeated freeze/thaw cycles.
重組大鼠成纖維細胞生長因子21蛋白
SDS-PAGE
Reference	1. Nishimura T, Nakatake Y, Konishi M, et al. 2000. Biochim Biophys Acta. 1492:203-6. 2. Kharitonenkov A, Shiyanova TL, Koester A, et al. 2005. J Clin Invest. 115:1627-35. 3. Kurosu H, Choi M, Ogawa Y, et al. 2007. J Biol Chem. 282:26687-95
Background	Fibroblast growth factor 21 (FGF-21) encoded by the FGF-21 gene belongs to the large FGF family and it is specifically induced by HMGCS2 activity. In mice, brown adipose tissue becomes a source of systemic FGF-21 after cold exposure. FGF-21 stimulates glucose uptake in differentiated adipocytes via the induction of glucose transporter SLC2A1/GLUT1 expression (but not SLC2A4/GLUT4 expression) and the activity depends on the presence of KLB. Recombinant rat FGF-21 contains 180 amino acids residues and show limited binding to heparin. In addition, rat FGF-21 respectively shows 81 % and 92 % a.a. identity to human and murine FGF-21, and it show activity on murine and human cells.

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產品名稱：重組大鼠成纖維細胞生長因子21蛋白