人亮氨酸豐富α2糖蛋白1(LRG1)化學發光**分析試劑盒
基本原理：
本試劑盒采用雙抗體夾心法。用抗人LRG1抗體包被于酶標板上，實驗時標本或標準品中的LRG1會與包被抗體結合，游離的成分被洗去。依次加入生物素化的抗人LRG1抗體和辣根過氧化物酶標記的親和素?？谷薒RG1抗體與結合在包被抗體上的人LRG1結合、生物素與親和素特異性結合而形成**復合物，游離的成分被洗去。加入發光底物混合液，發光底物在辣根過氧化物酶的催化下發出熒光，用化學發光**分析儀測定化學發光值（RLU），LRG1濃度與化學發光值之間呈正相關，通過繪制標準曲線求出標本中LRG1的濃度。
人亮氨酸豐富α2糖蛋白1(LRG1)化學發光**分析試劑盒描述：
| 英文名稱 | Human LRG1 (Leucine Rich Alpha-2-Glycoprotein 1) CLIA Kit | ||
| 中文名稱 | 人亮氨酸豐富α2糖蛋白1(LRG1)化學發光**分析試劑盒 | ||
| 貨號 | KL-H0838c | 種屬 | Human/人 |
| 規格 | 96T/Kit (8*12 wells) | ||
| 檢測方法 | 雙抗體夾心法 | ||
| 檢測范圍 | 1.563~100ng/mL | 靈敏度 | 0.938ng/mL |
人亮氨酸豐富α2糖蛋白1(LRG1)化學發光**分析試劑盒試劑盒組成：
| 中文名稱 | 英文名稱 | 規格 | 保存 |
| CLIA酶標板 | Micro CLIA Plate | 8×12 wells | 4℃/-20℃ # |
| 凍干標準品 | Reference Standard | 2 支 | 4℃/-20℃ # |
| 標準品&樣品稀釋液 | Reference Standard & Sample Diluent | 1瓶 20mL | 4℃ |
| 濃縮生物素化抗體 | Concentrated Biotinylated Detection Ab | 1支 120μL | 4℃/-20℃ # |
| 生物素化抗體稀釋液 | Biotinytated Detection Ab Diluent | 1瓶10mL | 4℃ |
| 濃縮HRP酶結合物 | Concentrated HRP Conjugate | 1支 120μL | 4℃(避光) |
| 酶結合物稀釋液 | HRP Conjugate Diluent | 1瓶 10mL | 4℃ |
| 濃縮洗滌液（25×） | Concentrated Wash Buffer (25×) | 1瓶 30mL | 4℃ |
| 發光底物A液 | Substrate Reagent A | 1瓶 5mL | 4℃(避光) |
| 發光底物B液 | Substrate Reagent B | 1瓶 5mL | 4℃(避光) |
| 封板覆膜 | Plate Sealer | 5 張 |
|
| 產品說明書 | Manual | 1 份 |
|
| 質檢報告 | Certificate of Analysis | 1 份 |
|
|
特別說明： #: 一周內使用可存于4℃，需長時間存放或多次使用建議存于-20℃. |
|||
1.在各孔中加入標準品或樣品各 100μL，37℃孵育90分鐘
2. 倒去孔內液體，拍干，加入 100μL 生物素化抗體工作液，37℃孵育 60 分鐘
3. 洗滌 3 次
4. 加入 100μL 酶結合物工作液， 37℃孵育 30 分鐘
5. 洗滌 5 次
6. 加入 100μL 發光底物混合液， 37℃孵育 5 分鐘左右
7. 立即測定各孔的化學發光值
8. 結果計算
KL-Flt3-Hu 人FMS樣酪氨酸激酶3(Flt3) 檢測試劑盒 human FMS Like Tyrosine Kinase 3 (Flt3) ELISA Kit Homo sapiens human 0.313-20ng/mL 0.108ng/mL 96T 12 months
KL-CX3CL1-Hu 人趨化因子C-X3-C-基元配體1(CX3CL1) 檢測試劑盒 human Chemokine C-X3-C-Motif Ligand 1 (CX3CL1) ELISA Kit Homo sapiens human 0.156-10ng/mL 0.053ng/mL 96T 12 months
KL-CX3CL1-Mu 小鼠趨化因子C-X3-C-基元配體1(CX3CL1) 檢測試劑盒 mouse Chemokine C-X3-C-Motif Ligand 1 (CX3CL1) ELISA Kit Mus musculus mouse 0.156-10ng/mL 0.060ng/mL 96T 12 months
KL-CX3CL1-Ra 大鼠趨化因子C-X3-C-基元配體1(CX3CL1) 檢測試劑盒 Rat Chemokine C-X3-C-Motif Ligand 1 (CX3CL1) ELISA Kit Rattus norvegicus Rat 0.156-10ng/mL 0.061ng/mL 96T 12 months
KL-NAP3-Hu 人中性粒細胞激活蛋白3(NAP3) 檢測試劑盒 human Neutrophil Activating Protein 3 (NAP3) ELISA Kit Homo sapiens human 15.625-1000pg/mL 6.5pg/mL 96T 12 months
KL-GCSF-Hu 人粒細胞集落刺激因子(GCSF) 檢測試劑盒 human Colony Stimulating Factor 3, Granulocyte (GCSF) ELISA Kit Homo sapiens human 15.625-1000pg/mL 5.4pg/mL 96T 12 months
KL-GCSF-Mu 小鼠粒細胞集落刺激因子(GCSF) 檢測試劑盒 mouse Colony Stimulating Factor 3, Granulocyte (GCSF) ELISA Kit Mus musculus mouse 15.625-1000pg/mL 5.1pg/mL 96T 12 months
KL-GDNF-Hu 人膠質細胞系來源神經營養因子(GDNF) 檢測試劑盒 human Glial Cell Line Derived Neurotrophic Factor (GDNF) ELISA Kit Homo sapiens human 0.156-10ng/mL 0.053ng/mL 96T 12 months
KL-GDNF-Ra 大鼠膠質細胞系來源神經營養因子(GDNF) 檢測試劑盒 Rat Glial Cell Line Derived Neurotrophic Factor (GDNF) ELISA Kit Rattus norvegicus Rat 78.125-5000pg/mL 29pg/mL 96T 12 months
KL-GH-Hu 人生長**(GH) 檢測試劑盒 human Growth Hormone (GH) ELISA Kit Homo sapiens human 0.156-10ng/mL 0.059ng/mL 96T 12 months
KL-GMCSF-Hu 人粒細胞巨噬細胞集落刺激因子(GMCSF) 檢測試劑盒 human Colony Stimulating Factor 2, Granulocyte Macrophage (GMCSF) ELISA Kit Homo sapiens human 15.625-1000pg/mL 6.4pg/mL 96T 12 months
KL-GMCSF-Mu 小鼠粒細胞巨噬細胞集落刺激因子(GMCSF) 檢測試劑盒 mouse Colony Stimulating Factor 2, Granulocyte Macrophage (GMCSF) ELISA Kit Mus musculus mouse 15.625-1000pg/mL 7.4pg/mL 96T 12 months
KL-GMCSF-Ra 大鼠粒細胞巨噬細胞集落刺激因子(GMCSF) 檢測試劑盒 Rat Colony Stimulating Factor 2, Granulocyte Macrophage (GMCSF) ELISA Kit Rattus norvegicus Rat 15.625-1000pg/mL 6.5pg/mL 96T 12 months
KL-gp130-Hu 人糖蛋白130(gp130) 檢測試劑盒 human Glucoprotein 130 (gp130) ELISA Kit Homo sapiens human 0.313-20ng/mL 0.115ng/mL 96T 12 months
KL-gp130-Mu 小鼠糖蛋白130(gp130) 檢測試劑盒 mouse Glucoprotein 130 (gp130) ELISA Kit Mus musculus mouse 125-8000pg/mL 48pg/mL 96T 12 months
KL-HGF-Hu 人肝細胞生長因子(HGF) 檢測試劑盒 human Hepatocyte Growth Factor (HGF) ELISA Kit Homo sapiens human 125-8000pg/mL 46pg/mL 96T 12 months
KL-ICAM1-Hu 人細胞間粘附分子1(ICAM1) 檢測試劑盒 human Intercellular Adhesion Molecule 1 (ICAM1) ELISA Kit Homo sapiens human 78.125-5000pg/mL 31pg/mL 96T 12 months
KL-ICAM1-Mu 小鼠細胞間粘附分子1(ICAM1) 檢測試劑盒 mouse Intercellular Adhesion Molecule 1 (ICAM1) ELISA Kit Mus musculus mouse 0.313-20ng/mL 0.134ng/mL 96T 12 months
KL-ICAM1-Ra 大鼠細胞間粘附分子1(ICAM1) 檢測試劑盒 Rat Intercellular Adhesion Molecule 1 (ICAM1) ELISA Kit Rattus norvegicus Rat 0.313-20ng/mL 0.113ng/mL 96T 12 months
KL-IFNg-Hu 人干擾素γ(IFNγ) 檢測試劑盒 human Interferon Gamma (IFNg) ELISA Kit Homo sapiens human 15.625-1000pg/mL 5.5pg/mL 96T 12 months
KL-PAF-Mu 小鼠血小板激活因子(PAF) 檢測試劑盒 mouse Platelet Activating Factor (PAF) ELISA Kit Mus musculus mouse 370.37-30000pg/mL 145.5pg/mL 96T 12 months
KL-FG-Ra 大鼠纖維蛋白原(FG) 檢測試劑盒 Rat Fibrinogen (FG) ELISA Kit Rattus norvegicus Rat 125-8000ng/mL 54ng/mL 96T 12 months
KL-SST-Ra 大鼠生長抑素(SST) 檢測試劑盒 Rat Somatostatin (SST) ELISA Kit Rattus norvegicus Rat 6.17-500pg/mL 2.16pg/mL 96T 12 months
KL-SST-Mu 小鼠生長抑素(SST) 檢測試劑盒 mouse Somatostatin (SST) ELISA Kit Mus musculus mouse 6.17-500pg/mL 2.36pg/mL 96T 12 months
KL-bEP-Ra 大鼠β-內啡肽(βEP) 檢測試劑盒 Rat Beta-Endorphin (bEP) ELISA Kit Rattus norvegicus Rat 12.35-1000pg/mL 4.35pg/mL 96T 12 months
KL-MTL-Hu 人胃動素(MTL) 檢測試劑盒 human Motilin (MTL) ELISA Kit Homo sapiens human 12.35-1000pg/mL 4.28pg/mL 96T 12 months
KL-CGRP-Hu 人降鈣素基因相關肽(CGRP) 檢測試劑盒 human Calcitonin Gene Related Peptide (CGRP) ELISA Kit Homo sapiens human 12.35-1000pg/mL 4.26pg/mL 96T 12 months
